Detection of NOEs/ROEs and/or chemical exchange between water molecules and protein protons
allows the precise location of the bound water molecules to be determined.
Excellent reviews in
Also see Hydrogen Bonding
NMR experiments to investigate water-protein interactions are generally
composed by three building blocks: i) Selective excitation of the water magnetization, ii) Magnetization transfer from water to protein protons, iii)Labeling of protein protons and final acquisition. Applications: